Both types of endothelial cells were exposed for 24 h to either 0.03 Pa or 1.5 Pa shear stress, corresponding to regions of low shear and high shear in the computational aneurysm model, respectively. A microfluidic device either cultured with human umbilical vein endothelial cells (HUVECs) or human induced pluripotent stem cell-derived endothelial cells (hiPSC-EC) was used. CT imaging was used to calculate CFD parameters using an immersed boundary method. In this study, the results of a personalized aneurysm-on-a-chip model using patient-specific flow parameters and patient-specific cells are presented. The pathophysiological mechanism of aneurysm formation is thought to be a consequence of blood flow (hemodynamic) induced changes on the endothelium. N2 - Intracranial aneurysms are pouch-like extrusions from the vessels at the base of the brain which can rupture and cause a subarachnoid hemorrhage.
M1 CHIP OUTPERFORMS COMPUTATIONAL FLUID DYNAMICS LICENSE
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( /licenses/by/ 4.0/). Contributions by Julia Mikhal were made possible on the basis of her NWO-VENI grant ‘Surgery or not? Multiscale prediction of developing vascular diseases’. T2 - Setting Flow Parameters for Microfluidic Endothelial Cultures Based on Computational Fluid Dynamics Modeling of Intracranial Aneurysmsįunding: A part of this study was made possible through ZonMW Grant No.: 40-4351435004025: “Modeling New Treatment Strategies for lntracranial Aneurysms”.Ī part of this study was made possible through ZonMW Grant No.: 40-4351435004025: “Modeling New Treatment Strategies for lntracranial Aneurysms”.Simulations were performed on the Cartesius computer at SARA in Amsterdam, enabled by grant SH061-Multiscale Modeling and Simulation, of NWO. Future research should focus on hiPSC-EC stabilization to allow personalized intracranial aneurysm models.", HiPSC-EC exhibited reduced cell numbers, monolayer gap formation and cells with aberrant, spread-out morphology. Under high shear conditions HUVEC elongated and aligned in the direction of the flow. Both HUVEC and hiPSC-EC cultures presented as confluent monolayers with no particular cell alignment in static or low shear conditions. As a control, both cell types were also cultured under static conditions for 24 h as a control. Future research should focus on hiPSC-EC stabilization to allow personalized intracranial aneurysm models.Ībstract = "Intracranial aneurysms are pouch-like extrusions from the vessels at the base of the brain which can rupture and cause a subarachnoid hemorrhage.
Intracranial aneurysms are pouch-like extrusions from the vessels at the base of the brain which can rupture and cause a subarachnoid hemorrhage.
0 kommentar(er)
